Electrophysiology Workshop

Neural organoids derived from human induced pluripotent stem cells (h-iPSCs) are becoming powerful tools for studying brain development and disease mechanisms. These 3D models and organoids provide non-invasive, rapid, patient-specific, and cost-effective approaches for drug discovery and disease modeling. As the field grows, there is a rising need for advanced technologies to assess the cellular composition, gene expression, and functional properties of different types of neural organoids.

MaxWell Biosystems’ High-Density Microelectrode Array (HD-MEA) technology enables researchers to capture neuronal activity at high resolution, without the need for labels. Both the MaxOne (single-well) and MaxTwo (multi-well) HD-MEA platforms allow precise observation, recording, and analysis of the electrophysiological behavior of electrogenic samples at the network, single-cell, and sub-cellular levels. These systems consistently provide robust, reproducible, and high-quality data across numerous scientific applications.

In this workshop, participants will:

  • Receive a step-by-step walkthrough on plating organoids onto the HD-MEA chip

  • See examples of high-resolution functional imaging of iPSC-derived models using the HD-MEA platform

  • Review results and analyses from iPSC-derived brain models representing different brain regions

  • Learn how HD-MEA technology advances the study of human iPSC-derived models and facilitates compound testing

The session will highlight practical applications of MaxWell Biosystems’ HD-MEA technology in monitoring the electrophysiological properties of brain organoids. Attendees will be guided through organoid plating on the HD-MEA chip and then introduced to datasets and analyses from previously recorded experiments. By the end of the workshop, participants will gain hands-on insights into acquiring reliable organoid data and evaluating functional properties across different samples with MaxWell Biosystems’ HD-MEA systems.

Neural Organoid Workshop

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3D Bioprinting Workshop

Creating Scalable, Biomimetic neuronal models using RASTRUM Drop-on-Demand Bioprinting

Current in vitro neuronal models are limited in their capacity to recreate the neuronal tissue environment and cellular complex 3D arrangement. This workshop provides a hands-on demonstration of how to use Inventia's novel bioprinting platform RASTRUM which addresses the limitation of 2D and suspension neuronal cultures by generating tissue-relevant, scaled 3D CNS models.

Participants will gain practical expertise in the complete workflow for creating complex 3D neuronal cultures at scale:

  • Informed in vitro model selection: select the in vitro model tailored to specific research questions, whether specific neuronal subtype mono culture, neuronal-glial co-cultures, or multi-region network and migration models.

  • Modelling the brain tissue extracellular matrix: We will demonstrate how to select RASTRUM matrix options for tissue-matched culture environments. The poly ethylene glycol-based hydrogel library contains extracellular conditions to provide an ideal environment for cell growth and differentiation over long culture periods.

  • Streamlined Bioprinting across well plates: Detailed use of the RASTRUM drop-on-demand printing platform to create highly reproducible hydrogel 3D cultures from mono to complex co-cultures for low and high throughput experiments.

  • Downstream Analysis: Finally, participants will learn the standardized methods for quantifying these advanced models, including immunostaining, functional assays, and molecular analysis.

By demonstrating this scalable 3D neuronal culture approach, this workshop will illustrate how bioprinted models can address the limitations of other 3D CNS systems by meeting crucial requirements for disease modeling and drug screening.


Genomics Workshop

Parsing Brain Organoids One Cell at a Time

Presented by SAGC and Decode Science

Join South Australian Genomics Centre (SAGC )and Decode Science for an in-depth workshop showcasing the capabilities of the Parse Biosciences single cell platform and its application to brain organoid research. As a certified Parse service provider, SAGC offers a complete workflow from single cell barcoding and library preparation to cost-effective sequencing using the MGI platform and downstream data analysis.

This session will highlight how Parse single cell transcriptomics technology differs from traditional droplet-based approaches, including itsspecies-agnostic chemistry and freedom from cell size limitations, making it ideally suited for neuron-like and organoid-derived cells. Attendees will gain insight into the workflow, experimental design, and budgeting considerations for Parse single cell projects with integrated MGI sequencing options.

The workshop will also feature a demonstration of Trailmaker, Parse’s intuitive cloud-based analysis platform, showcasing how it streamlines data analysis and visualization for single cell studies.

Whether you are new to single cell genomics or looking to enhance your organoid research, this session will provide practical guidance and examples from SAGC’s experience delivering Parse single cell and MGI sequencing services.

Live Imaging Workshop

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